Fig. 3: Purification of MaSp2 proteins from R. sulfidophilum transformants. | NPG Asia Materials

Fig. 3: Purification of MaSp2 proteins from R. sulfidophilum transformants.

From: Production of the recombinant spider silk MaSp2 protein using the marine purple photosynthetic nonsulfur bacterium Rhodovulum sulfidophilum under autotrophic conditions

Fig. 3: Purification of MaSp2 proteins from R. sulfidophilum transformants.

a Growth curve for R. sulfidophilum transformants in triplicate experiments. b Cell lysates disrupted by sonication were eluted through a Ni column and analyzed by SDS‒PAGE. Lane 1: cell lysate immediately before loading onto the Ni column. FT: flow-through. After imidazole passed through the column, the eluate was collected in 500 µL aliquots, and 10 µL of each aliquot was loaded into the wells from left to right in elution order. The three elution fractions marked with * were collected and subjected to ultracentrifugation. c Lane 1 represents the cell lysate before being applied to the Ni column, and lane 2 represents the protein mixture after being subjected to ultrafiltration (MWCO, 10,000). The left and right panels show Coomassie blue staining and Western blot results, respectively, for b and c. The black triangle indicates the 43 kDa band of the model MaSp2 protein.

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