Table 2 Analysis of cultures on the final day of cultivation used for MaSp2 purification by TOC or TN instruments, the amount of cells harvested, and the amount of MaSp2 collected.

From: Production of the recombinant spider silk MaSp2 protein using the marine purple photosynthetic nonsulfur bacterium Rhodovulum sulfidophilum under autotrophic conditions

 

TOC

TC

IC

TN

Used C

Used N

Cells

TSP

TSP

TSP

MaSp2 in TSP

MaSp2

MaSp2

MaSp2

 

(mg/L)

(mg/L)

(mg/L)

(mg/L)

(mg/L)

(mg/L)

(g, wet)

(µg/9 L)

(µg/L)

(µg/g wet cell)

(%)

(µg/9 L)

(µg/L)

(µg/g wet cell)

Culture

55.2 ± 28.0

373.4 ± 43.9

318.2 ± 31.6

10.8 ± 1.8

          

Supernatant

30.5 ± 23.3

338.4 ± 41.1

307.9 ± 29.8

5.3 ± 0.7

          
     

65.5 ± 27.9

5.4 ± 1.1

1.6 ± 0.7

192.7 ± 125.2

21.4 ± 13.9

141.8 ± 128.6

12.1 ± 9.0

28.6 ± 34.1

3.2 ± 3.8

24.6 ± 34.7

  1. Analysis of the culture medium or supernatant collected on the final day of incubation using the TOC/TN instrument, the values of C or N taken up into the cells, cell and MaSp2 yields in the culture and purification experiments. TSP: total soluble protein. Each value is the average ± SD of three independent experiments.
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