Fig. 4

LLPS phenomena in silk proteins. A LLPS propensity of MaSp1 N-R6-C (blue shading) and MaSp2 N-R6-C (red dotted line) in response to potassium phosphate, plotted as a function of the protein concentration (x-axis) and phosphate concentration (y-axis). Under similar conditions, MaSp1 exhibited a markedly higher tendency for phase separation than MaSp2. B LLPS of miniMiSp (40 mg/mL) upon addition of 10 mM Tris buffer (pH 7.0) and 100 mM NaCl. C Phase separation of B. mori silk fibroin (SF) as a function of protein and Ca²⁺ concentrations. Squares correspond to no LLPS observed, triangles correspond to LLPS observed after 15 min, and circles correspond to LLPS observed immediately after mixing. The data point inside the dashed box corresponds to the inset fluorescence images on the side of the graph (scale bar 50 µm). D Kyte-Doolittle hydropathy plots for the repetitive regions of A. ventricosus MaSp2, MaSp1, B. mori Fib-H and A. ventricosus MiSp. A higher hydrophobicity score might be related to a higher propensity for LLPS and chaotropic-induced LLPS. Panel (A) reprinted with permission from Reference [61]; copyright by John Wiley and Sons Ltd. Panel (B) reprinted with permission from Reference [106]; copyright by Elsevier. Panel (C) reprinted from [104] under the Creative Commons CC BY license