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Bioactive hydrogenated azaphilones from acid-tolerant fungus Penicillium purpureum OUCMDZ-019

The Journal of Antibiotics volume 78, pages 281–287 (2025) Cite this article

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Abstract

Two new hydrogenated azaphilones (1 and 2), together with the known azaphilone (3) were isolated from the red soil-derived acid-tolerant fungus Penicillium purpureum OUCMDZ-019 by OSMAC (one strain many compounds) strategy. Their structures were determined by nuclear magnetic resonance (NMR) spectroscopy analysis and electronic circular dichroism (ECD) calculations. Compound 1 was the first reported azaphilone that salified with pyridine and chlorination occurred at C-1, and it exhibited potential inhibitory activity on melanin production as tyrosinase inhibitor in vivo. Furthermore, (+)-mitorubrinol acetate (3) showed significantly inhibitory activity against H1N1 with IC50 values of 58.6 μM (ribavirin, IC50 85.0 μM) as the first report.

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (Nos. 82373748, 81973198, 82111540166, and U1906213).

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  1. These authors contributed equally: Chengfeng Hou, Yuqing Wang.

Authors and Affiliations

  1. School of Medicine and Pharmacy, Ocean University of China, Qingdao, China

    Chengfeng Hou, Yuqing Wang, Xiangling Meng, Ling Lu, Peng Fu, Weiming Zhu & Yi Wang

  2. Laboratory for Marine Drugs and Bioproducts, Qingdao Marine Science and Technology Centre, Qingdao, China

    Weiming Zhu & Yi Wang

  3. Key Laboratory of Marine Drugs, Ministry of Education of China, School of Medicine and Pharmacy, Ocean University of China, Qingdao, China

    Weiming Zhu & Yi Wang

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  1. Chengfeng Hou
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Hou, C., Wang, Y., Meng, X. et al. Bioactive hydrogenated azaphilones from acid-tolerant fungus Penicillium purpureum OUCMDZ-019. J Antibiot 78, 281–287 (2025). https://doi.org/10.1038/s41429-025-00818-7

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