Fig. 1: A subcutaneous adipose biopsy was performed in participants 10–15 wk after initiating low- (light bar), moderate- (gray bar), and high- (dark bar) carbohydrate diets with adjustment of energy intake for weight-loss maintenance.
From: A high-carbohydrate diet lowers the rate of adipose tissue mitochondrial respiration
Permeabilized adipose samples were sequentially treated with glutamate (10 mM) and malate (2 mM; GM), then ADP (2.5 mM; GMD), then succinate (2.5 mM; GMDS). Finally, FCCP was added (0.05 µM; FCCP). Mitochondrial respiration data (expressed in pmol O2/sec/µg protein) were calculated as change from first biopsy (pre-randomization, post-weight loss) to second biopsy (after 10–15 weeks on weight-loss maintenance diets), as described in Methods.
