Fig. 7: Analysis of chemokine levels in human PBMCs from females and males. | Gene Therapy

Fig. 7: Analysis of chemokine levels in human PBMCs from females and males.

From: Unveiling the sex bias: higher preexisting and neutralizing titers against AAV in females and implications for gene therapy

Fig. 7

PBMC from 4 male and 4 female donors were stimulated with AAV9 with 5 x 1010 particles/ml, 1 x 1011 particles/ml or vehicle for 48 h. Supernatant were analyzed for secretion of 10 human chemokines by multiplexed nELISA. A Fold change analysis of chemokine secretion. B Levels of individual chemokines: Chemokine (C-X-C motif) ligand 10 (CXCL10), CXCL9, CCL2, CXCL 1, CCL3, CCL7 (monocyte chemotactic protein3- MCP-3), CCL5 (RANTES), CCL4, CXCL 11 and CXCL 4 in female PBMCs compared to males. Data (n = 4 donors of each sex) are shown as Mean ± SEM on a Log 2 scale. Two-way ANOVA was performed, with * indicating p < 0.05, ** for p < 0.0005, and *** for p < 0.0001.

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