Fig. 4
From: Mutant RAMP2 causes primary open-angle glaucoma via the CRLR-cAMP axis
Lower cAMP levels in the Ramp2+/− retina treated with AM and RGC death were observed in the Ramp2+/−mice. (a,b) the cAMP levels were significantly lower in the RGC layer in the Ramp2+/− retina treated with AM compared with the WT mice (P = 0.01). The asterisk indicates a P < 0.05 by t-test. (c) RGC apoptosis (green) was observed in the Ramp2+/− retina, compared with negative staining in WT retina. The red arrows indicated detection of an apoptosis signal. Each experiment was replicated in triplicate. Nuclei were stained with 4’-6-diamidino-2-phenylindole (DAPI). INL inner nuclear layer, ONL outer nuclear layer, RGC retinal ganglion cell, WT wild-type. Scale bar: 20 μm.
