Table 1 TaqMan® qPCR assays for candidate genes (with putative function) with primer and probe sequences for assays include on the OpenArray® gene transcription chip. Reference gives the source for existing qRT-PCR assays, “This study” indicates new assays developed here.
Function | Name | Primers and Probes (5’-3’) | Reference |
|---|---|---|---|
Reference genes | Ribosomal protein lateral stalk subunit P0 (RPLP0) | F: AGGACCACGTGGAAGTCCAA | (Laborda 1991) |
R: CGCCGACAATGAAACATTTG | |||
P: TATGAAAATCATCCAATTGC | |||
Elongation factor 1A (EF1A) | F: AATACCCTCCTCTTGGTCGTTTC | (Laborda 1991) | |
R: CTTGTCGACGGCCTTGATG | |||
P: TGCGTGACATGAGGC | |||
Immune genes | Annexin-1 (ANXA1) | F: ACCTCCAGAAGTGCTCCTCA | This study |
R: ATAGCCTTTGCCACATCCAC | |||
P: CCGCCAAGCGTTT | |||
Complement C1q subcomponent subunit B precursor (C1qb) | F: CGTTTCGACGGACCAATACT | This study | |
R: TAAACAACCCGTCCTTCAGC | |||
P: TTGACCCTAGCCTGGAA | |||
Complement 3 (C3) | F: GAACAGCTGGAGGTCAAAGC | This study | |
R: TTCTCCATCAGCTCAACACG | |||
P: TCCACAACTACAGCGAAG | |||
Calmodulin (CALM2a) | F: CAGACAGCGAGGAGGAGATCA | (Wellband et al. 2018) | |
R: TAACCGTTCCCATCCTTGTCA | |||
P: AGAAGCGTTCCGTGTCT | |||
T-cell surface glycoprotein CD3 epsilon (CD3E) | F: TCAGGGCTCGGAAGAAGTCT | (Tadiso et al. 2011) | |
R: GCCACGGCCTGCTGA | |||
P: ACCTGCTGCACCC | |||
C-C motif chemokine 20 (CCL20) | F: TCCTGGCTGCTCTGTTCTCTCT | (Toews et al. 2019) | |
R: ACAGCAGTCCGCTGATTGTG | |||
P: CTCATCATCACCCTCATT | |||
Interleukin 8 (IL8) | F: CGCACTGCAGAGACACTGA | (Ching et al. 2010) | |
R: ACAAATCTCCTGACCGCTCTTG | |||
P: TCAGAGTGGCAATGATC | |||
Interleukin 1 (ILIB) | F: CCAGGGAGGCAGCAGCTA | (Ching et al. 2010) | |
R: CGGGCGTGACGTACGAA | |||
P: ACAAAGTGCATTTGAAC | |||
Immunoglobulin mu A2 (IGHA2) | F: CGCTGTAGATCACTTGGAAAACC | (Ching et al. 2010) | |
R: TCTCCTCCAGTCTCCCTCTTGT | |||
P: ACCTTGGTAAAGAAAGC | |||
Major Histocompatibility II beta (MHCII) (H2-AA) | F: GCCATACTGGACAAGACAGTTGAG | (Wellband et al. 2018) | |
R: TCATAGGCGCTGCACATCAG | |||
P: CCCATGTCAGACTGAG | |||
Natural Killer Enhancing Factor (NKEF) | F: TGAGGTCATTGGTGCCTCTGT | (Wellband et al. 2018) | |
R: GAGGTGTGTTGGTCCAAGCA | |||
P: ATTCCCACTTCTGCCATC | |||
Suppressor of Cytokine Signalling (SCS) | F: ACCTTTGCCGCATCACTATC | This study | |
R: TCCAGCAAAAAGTCCCGTAG | |||
P: CAGCATTCTCGCTGTCA | |||
B-cell lymphoma (BCL-2) | F: TTATGCCAGAGCTTTCAGTGAC | This study | |
R: ACCTCGTCCATCACGTTCTC | |||
P: TCCTCCCAGCTACACAT | |||
Genes with diverse functions including stress and grow | Transforming Growth Factor beta (TGFB) | F: CGTAACACAGACTCTGAATGAGTGG | This study |
R: ACAAGGCAACTTCAATTGGAATC | |||
P: TGCAAGGGGCTGGAGA | |||
Transferrin (TF) | F: GAAGGCATGCACTTTCCATT | This study | |
R: GTGTGTTTGCCACAAAGGTG | |||
P: CCAGCAGAAGGAAT | |||
Tumour Suppressor p53 (TS53) | F: CAGTCCAGCACAGCCAAGTC | (Toews et al. 2019) | |
R: CGCCAACTGGCAGAACAACT | |||
P: ACTTGCACATACTCGC | |||
Heat shock protein 70 (HSP70) | F: TCAACGATCAGGTCGTGCAA | (Wellband et al. 2018) | |
R: CGTCGCTGACCACCTTGAA | |||
P: CCGACATGAAGCACTG | |||
Metallothionein A (MetA) | F: GCTCCAAACTGGATCTTGCAA | (Wellband et al. 2018) | |
R: TGGTGCATGCGCAGTTG | |||
P: TGCGGTGGATCCTG | |||
Prostaglandin E synthase (PTGES) | F: AATACTGCGGTCTGGAGTGG | This study | |
R: AGACGGAGTCCCTTCTGTCA | |||
P: AAATAGATAGCAAGAGATGC |
