Table 4 Results of the LMM analysis for the effects of dam, sire, and dam × sire on host gut tissue gene transcription for 19 selected genes. Neither fish weight nor rearing tank ID random effects were significant (results not shown).
Function | Gene | Factors | DF | Sum Sq | F value | Pr(>F) |
|---|---|---|---|---|---|---|
Immune system | Annexin-1 (ANXA1) | Dam | 14 | 30.8 | 2.06 | 0.014 |
Sire | 10 | 12.08 | 1.22 | 0.27 | ||
Dam × Sire | 20 | 34.05 | 1.59 | 0.055 | ||
Complement C1q subcomponent subunit B precursor (C1qb) | Dam | 14 | 73.2 | 1.89 | 0.028 | |
Sire | 10 | 66.4 | 2.40 | 0.010 | ||
Dam × Sire | 20 | 62.2 | 1.12 | 0.32 | ||
Complement 3 (C3) | Dam | 14 | 27.5 | 0.72 | 0.74 | |
Sire | 10 | 15 | 0.55 | 0.84 | ||
Dam × Sire | 20 | 67.2 | 1.23 | 0.23 | ||
Calmodulin (calm2a) | Dam | 14 | 14.4 | 0.93 | 0.52 | |
Sire | 10 | 14.5 | 1.32 | 0.22 | ||
Dam × Sire | 20 | 19.6 | 0.89 | 0.59 | ||
T-cell surface glycoprotein CD3 epsilon (CD3E) | Dam | 14 | 32.2 | 1.89 | 0.028 | |
Sire | 10 | 23 | 1.90 | 0.046 | ||
Dam × Sire | 20 | 30.9 | 1.27 | 0.19 | ||
C-C motif chemokine 20 (CCL20) | Dam | 14 | 55.7 | 1.22 | 0.25 | |
Sire | 10 | 58.9 | 1.82 | 0.060 | ||
Dam × Sire | 20 | 50.4 | 0.77 | 0.73 | ||
Interleukin 8 (IL8) | Dam | 14 | 18.6 | 1.29 | 0.21 | |
Sire | 10 | 4.4 | 0.43 | 0.92 | ||
Dam × Sire | 20 | 15.4 | 0.74 | 0.77 | ||
Interleukin 1 (ILIB) | Dam | 14 | 27.8 | 0.67 | 0.8 | |
Sire | 10 | 37 | 1.24 | 0.26 | ||
Dam × Sire | 20 | 55 | 0.92 | 0.55 | ||
Immunoglobulin mu A2 (IGHA2) | Dam | 14 | 35.7 | 2.51 | 0.0026** | |
Sire | 10 | 53.3 | 5.24 | 0.000001*** | ||
Dam × Sire | 20 | 26.7 | 1.31 | 0.17 | ||
MHCII | Dam | 14 | 116 | 1.76 | 0.045 | |
Sire | 10 | 47.4 | 1.01 | 0.43 | ||
Dam × Sire | 20 | 98 | 1.04 | 0.41 | ||
Suppressor of Cytokine Signalling (SCS) | Dam | 14 | 16.4 | 1.11 | 0.34 | |
Sire | 10 | 9.8 | 0.93 | 0.50 | ||
Dam × Sire | 20 | 22.9 | 1.08 | 0.36 | ||
B-cell lymphoma (BCL-2) | Dam | 14 | 31 | 0.34 | 0.98 | |
Sire | 10 | 74.1 | 1.14 | 0.32 | ||
Dam × Sire | 20 | 87.3 | 0.67 | 0.84 | ||
Natural Killer Enhancing Factor (NKEF) | Dam | 14 | 5.7 | 0.29 | 0.99 | |
Sire | 10 | 5.9 | 0.42 | 0.93 | ||
Dam × Sire | 20 | 31 | 1.11 | 0.33 | ||
Diverse function (stress response and growth) | Transforming Growth Factor beta (TGFB) | Dam | 14 | 591.5 | 3.52 | 0.00001*** |
Sire | 10 | 352.6 | 2.93 | 0.0018** | ||
Dam × Sire | 20 | 377.8 | 1.57 | 0.062 | ||
Transferrin (TF) | Dam | 14 | 38.7 | 1.18 | 0.29 | |
Sire | 10 | 34.1 | 1.46 | 0.15 | ||
Dam × Sire | 20 | 63.9 | 1.36 | 0.14 | ||
Tumour Suppressor p53 (TS53) | Dam | 14 | 7.4 | 1.2 | 0.27 | |
Sire | 10 | 3.7 | 0.85 | 0.57 | ||
Dam × Sire | 20 | 10.3 | 1.16 | 0.28 | ||
Heat shock protein 70 (HSP70) | Dam | 14 | 20.4 | 1.71 | 0.05 | |
Sire | 10 | 9.7 | 1.14 | 0.33 | ||
Dam × Sire | 20 | 31.9 | 1.87 | 0.016 | ||
Metallothionein A (MetA) | Dam | 14 | 22.13 | 1.64 | 0.07 | |
Sire | 10 | 11.3 | 1.17 | 0.30 | ||
Dam × Sire | 20 | 36.7 | 1.90 | 0.013 | ||
Prostaglandin E synthase (PTGES) | Dam | 14 | 20.1 | 2.02 | 0.017 | |
Sire | 10 | 12.8 | 1.80 | 0.061 | ||
Dam × Sire | 20 | 23.8 | 1.67 | 0.040 |
