Fig. 2: The microinjection workflow, guideRNA locations, and scarlet knockout mutant phenotype. | Heredity

Fig. 2: The microinjection workflow, guideRNA locations, and scarlet knockout mutant phenotype.

From: The mosaicism of Cas-induced mutations and pleiotropic effects of scarlet gene in an emerging model system

Fig. 2: The microinjection workflow, guideRNA locations, and scarlet knockout mutant phenotype.The alternative text for this image may have been generated using AI.

A The microinjection workflow for CRISPR-Cas9/Cas12 RNP. B The schematic locations for the guide RNA targets for Cas9 and Cas12 gene editing. C The clear-eye phenotype (red arrow) caused by the knock-out of scarlet gene. D Wildtype animal.

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