Table 2 Summary of CRISPR-Cas12 experiments for the scarlet gene.
Experiment | No. of injected embryos | No. of hatching embryos (percentage) | No. of clear-eye Phenotype (percentage) | No. of mosaic phenotype (percentage) |
|---|---|---|---|---|
1. KO | 59 | 28 (47.5%) | 0 | 2 (7.1%) |
2. KO | 75 | 28 (37.3%) | 1 (3.6%) | 2 (7.1%) |
3. KO | 131 | 31 (23.7%) | 1 (3.2%) | – |
4. KO | 93 | 25 (26.7%) | 4 (16%) | – |
5. KO | 57 | 21 (36.8%) | 1 (4.7%) | 4 (19.0%) |
6. KO | 69 | 17 (24.6%) | 0 | 2 (11.8%) |