Fig. 3: Validation of the B chromosome assembly.
From: Genome assembly of Astatotilapia latifasciata uncovers B chromosome–linked chromatin reorganization
a, b Boxplots showing the distribution of log₂(2B/0B) Illumina read ratios and log₂(1B/0B) Hi-C read ratios across LG8, LG3, and Chr B, respectively, indicating B chromosome-specific enrichment. c Distribution of Hi-C reads mapped to the genome assemblies of 0B and 1B individuals. d Comparative coverage plot of Illumina reads mapped to the assembled B chromosome, with 0B (blue peaks) and 2B (red peaks) samples. Each peak represents read depth across 10 kb windows. Regions with substantial differences in coverage—lower in 0B and higher in 2B—are highlighted in gray. The X-axis indicates genomic position, and the Y-axis represents read depth. e Line plot comparing Hi-C interaction frequencies along the B chromosome between 0B (blue line) and 1B (red line) genomes, demonstrating increased interaction density in 1B. f, g Bar charts displaying the distribution of BLAST hits for B chromosome marker genes BncDNA and ihhb, confirming their enrichment on the B chromosome.
