Fig. 3: Characterization of BrOG1AOE mutants involved in soluble sugar metabolism in A. thaliana.
From: Brassica rapa orphan genes largely affect soluble sugar metabolism
a Sequence alignment of BrOG1A and BrOG1B. The coding sequences and protein sequences were obtained from the Brassica database (BRAD). The two solid black boxes represent synonymous mutations (132 bp, C to T) and nonsynonymous mutations (258 bp, G to T, causing E to D), respectively. b Phenotypes of BrOG1AOE T3 lines in A. thaliana. Representative images of 37-day-old mutants resulting from three independent genetic transformation events (T3-1, T3-2, and T3-3) and wild-type Col-0 (WT). The scale bars are 2 cm. c Soluble sugar content in 30-day-old wild-type (WT) and BrOG1AOE transgenic Arabidopsis plants. The analysis was performed in triplicate, with 15 plants per replicate. d Detection of enzyme activity of sucrose synthase (SUS) and invertase (INV). e Levels of AtSUS transcripts in WT and BrOG1AOE plants. d, e The analysis was performed in triplicate, with three plants per replicate. c–e All the values are expressed as the means ± SEs. The black letters indicate statistically different groups (one-way ANOVA, p < 0.05). c The red letters at the top of the bars indicate significant differences in total soluble sugars between the mutants and WT (one-way ANOVA, p < 0.05)
