Fig. 5: Chinese cabbage BrOG1 mutants complement the phenotype of BrOG1AOE Arabidopsis lines.
From: Brassica rapa orphan genes largely affect soluble sugar metabolism
a Phenotypes of BrOG1-knockout lines. The images were taken upon the appearance of four true leaves (30-day-old plants). Representative T2 plants (T2-6 and T2-19) from a T0 plant were self-pollinated (P2). GT-24 indicates wild-type plants. The scale bars are 3 cm. b Soluble sugar content in 30-day-old wild-type (GT-24) and BrOG1 mutants. The analysis was performed in triplicate, with fifteen plants per replicate. Fru (fructose, light blue bar), Glc (glucose, light green bar), and Suc (sucrose, light pink bar) were analyzed simultaneously. c Detection of the activity of sucrose synthase (SUS) and invertase (INV) using an ELISA. d Phylogenetic relationships of SUSs between A. thaliana and B. rapa. Thirteen amino acid sequences were analyzed. A neighbor-joining tree was constructed by aligning the amino acid sequences. The red solid triangle and green solid box indicate BrSUSs and AtSUSs, respectively. e Expression levels of BrSUSs in GT-24 and BrOG1 plants. c, e The analysis was performed in triplicate, with three plants per replicate. GT-24, control plants (light brown bar); T2-6 (light red bar); T2-19 (light orange bar). b, c, e All the values are expressed as the means ± SEs. The black letters indicate significant differences between the mutants and the control (one-way ANOVA, p < 0.05). b The red letters at the top of the bars indicate significant differences in total soluble sugars between the mutants and the control (one-way ANOVA, p < 0.05)
