Fig. 4

The relationship between AMPK and Akt. Role of Akt in the AMPK/Akt/eNOS signaling pathway in endothelial cells. HUVECs were exposed to LY294002 (30 μM) for 30 min before the administration of MK for 5 min. Then, the phosphorylation of AMPK (A) and eNOS (B) was analyzed by western blotting. The role of AMPK in the AMPK/Akt/eNOS signaling pathway in endothelial cells. HUVECs were exposed to compound C (30 μM) for 30 min before the administration of MK for 5 min. Then, the phosphorylation of Akt (C) and eNOS (D) was analyzed by western blotting. The results are shown as the means ± SEM (n = 3). #P < 0.05 vs. the control and *P < 0.05 vs. the MK group