Fig. 4

Increased AT₁R expression in aortas from Snx1^−/− mice. a AT₁R protein levels were upregulated in aortas from Snx1^−/− mice (*P < 0.05 vs. WT, n = 5). b Immunohistochemical staining of AT₁R revealed higher expression of AT₁R in Snx1^−/− mice than in WT mice (these studies were repeated three times; NC negative control; scale bar = 50 μm). c AT₂R expression was not changed in the aortas from Snx1^−/− mice, as analyzed by immunoblotting (n = 5). d D₅R expression was not changed in the aortas from Snx1^−/− mice, as analyzed by immunoblotting (n = 3). e Colocalization of AT₁R and SNX1 detected by immunofluorescence laser confocal microscopy. Representative images suggesting the colocalization of AT₁R and SNX1 in aortic sections from WT mice (scale bar = 50 μm) (e1). Colocalization was quantitatively analyzed with the ImageJ Colocalization Finder plugin, and Pearson’s correlation coefficient (Rr) and the overlap coefficient (R) were calculated (e2). f Immunoprecipitation assay results indicating the interaction between AT₁R and SNX1 in the aortas from WT mice (these studies were repeated three times; NC negative control; PC positive control)