Fig. 3
Interference with NRF1 downregulated ACE and ET-1 under both hypoxia and normoxia. HUVECs were transfected with NRF1 siRNA and cultured for 48 h. After hypoxia (1% O2, 24 h) or normoxia (20% O2, 24 h) treatments, interference efficiency was detected using western blot analysis (A, B). C, D The expression of NRF1, ACE, and ET-1 was measured through qRT-PCR (mean ± SD, *p ≤ 0.05, **p ≤ 0.01 by Student’s t test). E, F Cells were fixed and stained with anti-NRF1/ACE or anti-NRF1/ET-1 antibodies. The total intensity of each protein in single cells was quantified using ImageJ. At least 30 cells were quantified in each treatment group (mean ± SD, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 by Student’s t test)
