Fig. 4

Comparison of GCaMP6f in input and output in separate preparations. a Frame subtraction maps of activity from three different preparations expressing GCaMP6f in the input olfactory receptor neurons (OMP-GCaMP6f mice). The largest glomerular signals (ΔF/F) for each preparation are from low to high concentration (left) 0.6%, 2.6%, 5.6%, 10%; (middle) 4%, 5.9%, 9%, 11%; (right) 1.2%, 4.4%, 6.5%, 7.2%. b Frame subtraction maps of activity from three different preparations expressing GCaMP6f in the output (Pcdh21-Cre or Thy1-GCaMP6f mice). The largest glomerular signals (ΔF/F) for each map are from low to high concentration: (left) 10%, 27%, 46%, 59%; (middle) 27%, 29%, 45%, 52%; (right) 15%, 38%, 44%, 51%. c Mean spatial correlation of each odor concentration frame subtraction map with the maps from each of the other odor concentrations. Correlation differences were assessed using a Mann-Whitney U-Test: 11 vs. 1.83% (U = 66, p = 0.07), 11 vs. 0.36% (U = 34, p = 0.001), 11 vs. 0.12% (U = 58, p = 0.03), 1.83 vs. 0.36% (U = 40, p = 0.003), 1.83 vs. 0.12% (U = 50, p = 0.01), 0.36 vs. 0.12% (U = 55, p = 0.02). d Normalized amplitude vs. odor concentration. The thin lines are from the example preparations in panels a and b. The thick lines are averages that include input measurements from 11 hemibulbs in 7 preparations, and output measurements from 22 hemibulbs in 18 preparations (Pcdh21-Cre: 14 hemibulbs from 14 preparations including the 7 preparations in Fig. 3b; Thy1-GCaMP6f: 8 hemibulbs from 4 preparations, including the preparation in Supplementary Fig. 3). The error bars represent s.e.m., *p < 0.05; **p < 0.01 (Kruskall-Wallis Test, Mann-Whitney U-Test). Scale bars in a, b, 250 µm. ant, anterior; lat, lateral