Fig. 5

Output maps are relatively concentration invariant, but odorant specific. a Maps of the output in response to the two odorants methyl valerate and 2-heptanone presented at a range of concentrations. The largest signals (ΔF/F) from lowest to highest concentration for methyl valerate: 17%, 22%, 27%, 46%; 2-heptanone 13%, 22%, 50%. b Correlation of output maps evoked by the same odorant across a range of concentrations. c Correlation of the output maps evoked by two different odorants across a range of concentrations. This example is from a Thy1-GCaMP6f transgenic mouse in which GCaMP6f is selectively expressed in bulb output neurons²⁷ (Supplementary Fig. 1g–i). Similar results were obtained in two other preparations using Pcdh21-Cre transgenic mice with AAV1 GCaMP6f injections when comparing methyl valerate to isoamyl acetate, or methyl valerate to 2-hexanone. Scale bar in a 250 µm. ant, anterior; lat, lateral