Fig. 1

CIB2 is localized in the auditory hair cell stereocilia. a Structure of the wild-type, the Cib2 ^F91S and the Cib2 ^{tm1a(EUCOMM)Wtsi} (Cib2 ^tm1a, EUCOMM) alleles. b X-gal staining to document the Cib2 locus activity in the organ of Corti of P12 Cib2 ^tm1a/+ mice (left). Immunolocalization of β-galactosidase (green) in Cib ^2tm1a/+ mouse cochlea at P12 (right). The cochlear actin is labeled with rhodamine–phalloidin (red). c, d Confocal images of IHC (c) and OHC (d) stereocilia bundles immunostained with CIB2 antibody (middle panels in c and red in the merge panels), and actin was labeled with phalloidin (left panels in c and green in the merge panels) in the control Cib2 ^tm1a/+, Cib2 ^tm1a/tm1a, and Cib2 ^F91S/F91S mice at P12. Note that Cib2 truncation causes disappearance of CIB2 protein from stereocilia, while p.F91S mutation does not. e CIB2 localization in the rat IHC stereocilia at P10. f CIB2 localization at P12 in Cib2 ^F91S/+ OHCs. Scale bars: b 10 μm, c–f 5 μm