Fig. 7
From: CIB2 interacts with TMC1 and TMC2 and is essential for mechanotransduction in auditory hair cells
CIB2 interacts with TMC1 and TMC2. a Fluorescent images of cells co-expressing LHFPL5-GFP, TMIE-GFP, CIB2-GFP (positive control), TMC1-EGFP or TMC2-EGFP (donor) and CIB2-V5 (acceptor) before and after acceptor photobleaching within the indicated region (white box). For negative control, we used TMC1-EGFP (donor) and CIB1-V5 (acceptor). FRET efficiency images are calculated as E FRET = 100 × (I Post−I Pre)/I Post, where I Pre and I Post are the EGFP pixel intensities before and after acceptor bleaching, respectively. Scale bars: 10 µm. b Quantitative analysis of FRET efficiency in CHO-K1 cells co-expressing LHFPL5-GFP, TMIE-GFP, CIB2-GFP, TMC1-EGFP or TMC2-EGFP (donor) and CIB2-V5 (acceptor). Quantitative analysis of negative control [TMC1-EGFP (donor) and CIB1-V5 (acceptor)] is also shown. Asterisks indicate statistical significance of FRET efficiency (p ≤ 0.0001). Error bars represent SEM. NS: not significant
