Fig. 1
From: AIM1 is an actin-binding protein that suppresses cell migration and micrometastatic dissemination
AIM1 interacts with the actin cytoskeleton. Lysates from HEK293 cells transiently transfected with control or biotin-affinity-tagged AIM1 vectors from a single experiment were enriched by streptavidin beads. Co-precipitating complexes were eluted and eluates were separated by PAGE or analyzed by mass-spectrometry. a Representative image of a silver-stained gel of control and AIM1 biochemical enrichment experiments. Note the strong enrichment of a 42 kDa protein representing β-actin. b Endogenous AIM1 interacts with affinity-tagged β-actin in normal prostate epithelial cells (RWPE-1). Representative gel of three independent experiments is shown. c Pulldown of endogenous β-actin shows robust co-precipitation of AIM1 in RWPE-1 cells. Two percent of TCL was loaded as control. IB immunoblot, IP immunoprecipitate, TCL total cell lysate
