Fig. 7
From: Acetylation-induced TDP-43 pathology is suppressed by an HSF1-dependent chaperone program
An HSF1-dependent cascade suppresses TDP-43 acetylation-mimic-induced pathology in vivo in skeletal muscle. a Immunofluorescence analysis was performed on TA muscles co-electroporated with GFP-tagged TDP-43-ΔNLS-K145Q in combination with either control vector (pCDNA), an HSF1-specific siRNA, active HSF1 or inactive HSF1 (R71G) for 4 days. b Higher magnification ×40 images of muscles described in a are shown. Reduced levels of aggregated TDP-43 were observed in the presence of active HSF1 (red). c Adjacent myofibers in the presence (red) or absence (white asterisk) of active HSF1 are depicted. Reduced levels of aggregated TDP-43 were observed in the myofiber expressing active HSF1. d TDP-43 average aggregation intensity was quantified among the total HSF1-expressing muscle fibers. Error bars represent SEM, and the triple asterisk indicates statistical significance with ***p-value < 0.001 as measured by Student’s t-test from N = 3 biological replicates. ns = not significant. Scale bar = 50 μm
