Fig. 4
From: Negri bodies are viral factories with properties of liquid organelles
Effect of cytoskeleton-disrupting drugs on formation and evolution of NBs. Nocodazole (NCZ, 2 µM), Taxol (1.25 nM) and Cytochalasin D (Cyto D, 2.5 µM) were added 1 h before and kept all along infection. BSR cells were infected with CVS strain at a MOI of 0.5 and fixed at 16 h p.i. NT: non treated cells. a Confocal analysis was performed after staining with a mouse monoclonal anti-N antibody followed by incubation with Alexa-488 donkey anti-mouse IgG. DAPI was used to stain the nuclei. Scale bars correspond to 15 µm. b–d Quantification of cytoplasmic structures labeled with anti-N antibody in treated and non-treated cells. The number of small dots (surface < 0.26 µm2) b, of intermediate inclusions (surface between 0.26 and 3.7 µm2) c, and of large inclusions (surface >3.7 µm2) d per cell was quantified using the image toolbox of MatLab software as described in the experimental procedures. **p < 0.01, ns: not significant (n = 20, two tailed Mann Whitney U test). e Average surface of the largest inclusion in the cell 16 h p.i. in non-treated and treated cells. Areas of inclusions were determined using the image toolbox of MatLab software as described in the experimental procedures. The mean is shown with error bars representing the SD. ns: not significant; **p < 2.10−3; ***p < 10−3 (n = 20, two tailed Welch’s t-test)
