Fig. 10

Deletion of Yap1 in fetal trabecular myocardium does not result in thin compact myocardium. a Schematic figure showing generation of Nppa-CreER knock-in allele. b Whole-mount fluorescence view of E15.5 Nppa-CreER;R26-tdTomato heart. Tamoxifen was injected at E12.5. c Immunostaining for tdTomato and TNNI3 on heart section showed Nppa-CreER mainly targets trabecular myocardium. d Schematic figure showing strategy for Yap1 knockout and experimental design. e Immunostaining for YAP1 and TNNI3 on control or mutant heart sections shows a significant reduction of YAP1⁺ cardiomyocytes (arrowheads) in the mutant compared with the control. f Immunostaining for pHH3, TNNI3 and PECAM on the E15.5 mutant or control heart sections. g Quantification of the percentage of pHH3⁺ cardiomyocytes in trabecular myocardium of the control and mutant heart samples. *P < 0.05; n = 4. h Hematoxylin and eosin (H&E) staining of heart sections from P7 control and mutant hearts. Inserts indicate whole-mount images of hearts. Scale bars, 1 mm in h; 200 µm in b; 100 µm in c, e, f