Fig. 7
From: Identification of a hybrid myocardial zone in the mammalian heart after birth
Sema3a+ cells contribute to the innermost layer of ventricle wall in postnatal heart. a Schematic showing strategy for generation of Sema3a-CreER knock-in allele by homologous recombination. b Experimental strategy for tamoxifen administration and tissue analysis. c Immunostaining for ESR and TNNI3 on E12.5 and E13.5 Sema3a-CreER hearts. Most Sema3a+ (ESR+) cells were detected in inner trabecular layer (yellow arrowheads) but not in outer trabecular layer (white arrowheads). d, e Immunostaining for tdTomato and TNNI3 on heart section from P0, P7, and P8w (8 weeks) Sema3a-CreER;R26-tdTomato hearts. Sema3a-derived cells are restricted in the innermost layer of ventricle wall (IMW) in postnatal heart. f Cartoon image showing cell fate of Sema3a+ cells in postnatal heart. Scale bars, 100 µm in c; 500 µm in d, e; Each image is representative of four individual samples
