Fig. 3
From: Activated protein C protects from GvHD via PAR2/PAR3 signalling in regulatory T-cells
T-cell preincubation with aPC ameliorates GvHD in mice. a, b Recipient BALB/c mice were lethally irradiated (11 Gy) and transplanted with 5 × 106 BM and 0.5 × 106 T-cells without (B6T + BM) or with (B6T(aPC) + BM) aPC-preincubation (20 nM, 1 h, 37 °C) from donor C57BL/6 wt mice. Recipient mice were monitored for survival (a Kaplan–Meier curve) and physical parameters (b clinical score); pooled data from three independent experiments each with four recipients per group. c, d Photomicrographs (c) depicting typical morphology in liver, small intestine (SI), colon, and skin and bar graph (d) summarizing histological disease score. e, f In separate experiments recipients were euthanized 2 weeks post transplantation, splenic T-cells were harvested and stained for H2b, CD4, T-bet, ROR-γt, or FOXP3 (e) or H2b, CD4, IFN-γ, TNF-α, IL-17, or IL-10 (f) and analysed by flow cytometry; pooled data from three independent experiments each with four recipients per group. g Cytokines (IFN-γ, TNF-α, IL-17, IL-6, IL-10, and TGFβ1) were measured by ELISA in mouse plasma samples (N = 12 per group) collected from recipient mice euthanized 2 weeks post transplantation. Mean value ± SEM (d–g); haematoxylin and eosin stained sections (c size bar: 50 µm); MFI and exemplary FACS images corresponding to Fig. 3e, f are shown in Supplementary Figs. 2 and 3; *P < 0.05, **P < 0.01, ***P < 0.001 (a log-rank test; b ANOVA; d–g: t-test)
