Fig. 5
From: Every-other-day feeding extends lifespan but fails to delay many symptoms of aging in mice
Hematological and immunological analyses. a–j Number and size of the different blood cell types were determined using a hematology analyzer (two-way ANOVA; young + AL: n = 16, young + EOD: n = 16, old + AL: n = 20, old + EOD: n = 22). a RBC count, b hemoglobin concentration, c HCT, d MCHC, e RDW, f platelet count, g platelet volume, h PDW, i large cell ratio of platelets (>12 fl), and j PCT. k–w Peripheral blood leukocytes were analyzed using a ten-color flow cytometer (two-way ANOVA; young + AL: n = 16, young + EOD: n = 16, old + AL: n = 20, old + EOD: n = 22). Only CD45-positive cells were included in the analysis. k T cells (CD3+CD5+), l Ly6C+ T cells, m B2 cells (CD5−CD19+B220+), n CD11b+ B cells (CD19+B220+), o Ly6C+ B cells (CD19+B220+), p natural killer (NK) cells (CD3−CD5−NKp46+ and/or NK1.1+), q CD11b+ NK cells, r CD11c+ NK cells, s NKT cells (CD3+CD5+NKp46+ and/or NK1.1+), t granulocytes (CD11b+Ly6G+), u Ly6C+ monocytes (CD11b+), v Ly6C− monocytes (CD11b+), w non-specific cells, x plasma concentration of IgE immunoglobulins. AL ad libitum, EOD every-other-day feeding. Data are shown as mean ± SEM. Full data sets of hematological and immunological analyses are presented in Supplementary Tables 14 and 15
