Fig. 7

Histopathology. Representative images of sections derived from the a brain (scale bar = 20 µm), b thyroid gland (scale bar = 500 µm), c adrenal gland (scale bar = 500 µm), d liver (scale bar = 100 µm), e–g kidney (in e, f: scale bar = 200 µm; in g: scale bar = 100 µm), h testis (scale bar = 500 µm), and i lung (scale bar = 200 µm) are shown on the left. Quantification of a proportion of animals bearing eosinophilic thalamic inclusions (indicated by arrows), b median follicle size of the thyroid gland, c relative surface area covered by lipofuscin deposits within the adrenal gland, d number of hepatic microgranulomas (indicated by arrows), e number of urinary casts in the kidney (indicated by arrows), f number of hyaline changes in renal arterioles (indicated by arrows), g the relative surface area on renal sections covered by tubular luminal space, h ratio of testicular seminiferous epithelium/lumen in seminiferous tubules, and i coverage of broncheoli-associated lymphoid tissues (BALT) in the lung (indicated by arrows) are presented in panels on the right. Data in b are shown as median ± SEM and data in c–i are presented as mean ± SEM. Statistical analyses was performed using Fisher’s exact test for a or two-way ANOVA for b–i (young + AL: n = 16, young + EOD: n = 16, old + AL: n = 19, old + EOD: n = 20). AL ad libitum, EOD every-other-day feeding