Fig. 4
From: Thrombopoiesis is spatially regulated by the bone marrow vasculature
The inter-bone space is fully vascularized and contains MKs. a Reconstruction of BM along with bone structures for different types of bone revealed that the entire BM contains MKs (green, GPIX) and blood vessels (red, CD105). Grid square = 200 µm. b Quantitative analyses revealed that bone-to-bone distances differ between femur diaphysis (dia.) on the one hand and femur epiphysis (epi.), sternum, skull on the other hand. However, the vessel-to-vessel distances c and the percentage of vessel associated MKs d are comparable. MKs were grouped in bone associated (BA) or non-bone associated (NBA, more than 100 µm distance from the bone cortex) as depicted schematically e. A subsequent analysis of MK parameters for femur f or sternum g revealed that the percentage of vessel-associated MKs, the MK distance to the vessel of non-vessel-associated MKs or MK diameters were similar between BA and NBA MKs in both types of bone. Bar graphs represent mean ± SD; n = 4; ***p < 0.001 (Mann–Whitney U test)
