Fig. 2
Hypoxia reduces inflammatory processes and induces autophagy in the DSS mouse model of colitis. a, b, c, d, e, f and g WT and Nrlp3 −/− mice were administered with DSS or DSS-free water and subjected to normoxia (N, 21% O2) or hypoxia (H, 8% O2). After 18 h, mice were killed and colon biopsies were collected. Statistical analysis was performed using one-way ANOVA followed by Tukey’s post-test. Results represent mean + s.e.m., WT mice under normoxia: n = 5, WT mice under normoxia: n = 5, DSS-treated WT mice under normoxia: n = 6; Nrlp3 −/− mice under normoxia: n = 5; DSS-treated Nrlp3 −/− mice under normoxia: n = 6; WT mice under hypoxia: n = 4, DSS-treated WT mice under hypoxia: n = 5; Nrlp3 −/− mice under hypoxia: n = 3; DSS-treated Nrlp3 −/− mice under hypoxia: n = 3, *P < 0.05; **P < 0.01; ***P < 0.001. h Total protein was isolated and western blot performed. LC3-I and LC3-II bands were quantified, and autophagy was measured by variations in the ratio of LC3-II/LC3-I and the total amount of LC3 (LC3-I plus LC3-II) relative to β-actin
