Fig. 7
DMOG reduces inflammation and induces autophagy in DSS-treated mice. a, b, c, d, e and f WT mice were administered with 2% DSS for 5 days and co-administered with 8 mg DMOG injected intraperitoneally every second day. Weight loss was monitored during the course of treatment a. After a recovery period, mice were killed at day 9 colon biopsies were collected and colon length was measured b. Mucosal damage was assessed by colonoscopy c and murine endoscopic score of colitis severity (MEICS) was calculated d. H&E staining of distal colon sections displayed a significant decrease in barrier breakdown and inflammatory infiltrate in DMOG-treated mice e. Scale bar, 100 µm. Total histological score at day 9 was calculated as the sum of epithelial damage and infiltration score f. g, h, i, j, k, l and m distal colon biopsies were collected and transcript analysis was performed. Statistical analysis was performed using Student t-test.Results represent mean + s.e.m., DSS-treated mice administered with PBS: n = 5; DSS-treated mice administered with DMOG: n = 5; *P < 0.05; **P < 0.01. n Total protein was isolated and Western blot performed. LC3-I and LC3-II bands were quantified, and autophagy was measured by variations in the ratio of LC3-II/LC3-I and the total amount of LC3 (LC3-I plus LC3-II) relative to β-actin
