Fig. 3 | Nature Communications

Fig. 3

From: Membrane-free culture and real-time barrier integrity assessment of perfused intestinal epithelium tubes

Fig. 3

Barrier integrity assay in OrganoPlate. A fluorescent dye is inserted in the channel comprising the tube. Integrity of the tube barrier is quantified by measuring the amount of dye that is leaking out of the tube into the adjacent gel channel. a–c Sketch in vertical cross section showing fluorescence distribution: a in absence of a tube, b for the case of a leak-tight tube and c for a leaky tube. d–i Fluorescent images of microfluidic chips perfused with fluorescent molecules show experimental results for: gel only (d, g), leak-tight tube (e, h), and leaky tube (f–i) using both 150 kDa FITC-dextran and 4.4 kDa TRITC-Dextran during the same experiment

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