Fig. 2 | Nature Communications

Fig. 2

From: Unraveling a tumor type-specific regulatory core underlying E2F1-mediated epithelial-mesenchymal transition to predict receptor protein signatures

Fig. 2

Invasive potential and EMT marker expression in less-invasive and invasive human bladder and breast cancer cell lines. a Boyden chamber assay and western blot showing the invasive potential and the expression of E2F1 and EMT markers of various bladder and breast cancer lines. RT-4 was used as reference. b Indicated cells were transduced with adenoviral vector expressing the 4-OHT responsive estrogen receptor (ER)-E2F1 fusion protein (Ad.ER-E2F1) to conditionally activate E2F1 nuclear translocation. After 24 h they were applied to Boyden chamber assay and induced with 4-OHT or ethanol as control (left panels) or harvested for protein isolation and western blotting (right panels). c Cells were transduced with adenoviral vector expressing shE2F1 or shcontrol. After 72 h cell invasion was determined (left). Protein level and shE2F1 knockdown is indicated by immunoblots (right). All figures are representatives of at least three independent experiments. Error bars indicate s.e.m., n.d. not detectable

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