Table 2 Comparison of structurally equivalent residues involved in catalysis in pols β and μ
From: Time-lapse crystallography snapshots of a double-strand break repair polymerase in action
Pol β | Function | Pol μ | Function |
|---|---|---|---|
Ser180 | Binds β/γ phosphate | Gly320 | Binds β phosphate |
Arg183 | Binds β phosphate | Arg323 | Binds β phosphate |
Arg149 | Binds γ phosphate | Lys325 | Binds γ phosphate |
Gly189 | Binds γ phosphate | His329 | Binds γ phosphate |
Asp190 | Metal coordination | Asp330 | Metal coordination |
Asp192 | Metal coordination | Asp332 | Metal coordination |
Arg254 | Stabilizes Asp256 and PTa | Arg416 | Stabilizes Asp418 and PT |
Asp256 | Coordinates PT and Mec b | Asp418 | Coordinates PT and Mec |
Phe272 | Modulates Arg258/Asp192 | Trp434 | Stabilizes sugar of PT |
Asp276 | Stabilizes NTc base | Lys438 | Stabilizes NT base |
