Fig. 1 | Nature Communications

Fig. 1

From: Measuring inter-protein pairwise interaction energies from a single native mass spectrum by double-mutant cycle analysis

Fig. 1

Double-mutant cycle for measuring inter-protein pairwise interactions. X and Y stand for two proteins and i and j for two residues in these respective proteins that are mutated to some other residue (e.g., alanine) designated by ‘m’. The binding constants corresponding to the formation of the four complexes in the cycle and the free energies changes upon the mutations are also indicated in the scheme

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