Fig. 7
Knockdown of CBX4 and CBX6 promotes MEF reprogramming. a AP staining of iPSC colonies after OSKM induction in MEF cells infected with Cbx4, Cbx6, Cbx7, Ring1a, or control shRNA lentivirus, **p < 0.01 compared to control shRNA. b Sox2, Nanog, and Oct4 mRNA expression levels during MEF reprogramming with Cbx4, Cbx6, Cbx7, Ring1a, or control shRNA lentivirus, **p < 0.01 compared to control shRNA. c Proposed role of USP26 in ESC differentiation and MEF reprogramming. USP26 removes Ub from CBX4 and CBX6 for stabilization. Accumulated CBX4 and CBX6 bind to promoters of pluripotency genes, such as Sox2, Nanog, and Polycomb genes, such as Cbx7. The data are presented as means ± SD from four independent experiments. a, b Two-way ANOVA for multiple comparisons
