Fig. 1

Bm inhibition by Bs is mediated by the WapA toxin. a Bs (PY79, wt) and Bm (OS2) were grown separately or in a mixture in LB medium at 37 °C. Cells were plated for CFUs at the indicated time points. Each point represents an average value and s.d. of three independent experiments. b Representative time lapse microscopy images of (1) Bm (OS2), (2) Bs (AR16: amyE::P _rrnE -gfp), (3) a mixture of Bm (OS2) and Bs (AR16), and (4) a mixture of Bm (OS2) and Bs (OS24: ΔwapA, sacA::P _veg-mCherry ). Shown are overlay fluorescence from GFP or mCherry (green) and phase contrast (red) images, captured at the indicated time points. Bm cells are shown in black while Bs cells are shown in green. Scale bar represents 5 µm. c Bs (OS16::ΔwapA) and Bm (OS2) were grown separately or in a mixture and processed as in a. Each point represents an average value and s.d. of three independent experiments