Fig. 6

WapA delivery is not a prerequisite for Met extraction. a, b Bm (OS2) was grown over night in S7 medium in the presence (a) or the absence (b) of Met (50 μg/ml). Cells were then washed in PBSx1, mixed with Bs (OS25: ∆metE, ∆wapA) and resuspended in S7 supplemented with AHA (1 mM). Cells were incubated for additional 1.5 h, underwent click reaction and visualized by fluorescence microscopy. Shown are overlay images of fluorescence from AHA (green) and phase contrast (red; left panels), and AHA fluorescence alone (right panels). Scale bar represents 5 µm