Fig. 4

SIRT7 inhibits breast cancer motility. a, b Wound healing assay of SIRT7 KD T47D cells a and SIRT7-overexpressing BT549 cells b. Scale bar, 100 µm. c Representative images of Transwell invasion assay for SIRT7-overexpressing breast cancer BT549 and MDA-MB-231 cells. Cell number was counted in six randomly captured pictures. Invasive ability was normalized to control. Scale bar, 100 µm. d Wound healing assay in BT549 cells expressing ectopic SIRT7 in the presence or absence of TGF-β1 (5 ng/ml). Scale bar, 100 µm. e Immunoblots showing Col1a1 and α-SMA in F2S human fibroblast cells treated with Scramble or si-SIRT7. f, g Quantitative RT-PCR and immunofluorescence staining of α-SMA in the Scramble (Scr) or si-SIRT7-treated F2S cells in the presence or absence of TGF-β1 (5 ng/ml). Scale bar, 50 µm. Data are shown as mean ± S.E.M. *P < 0.05, **P < 0.01. P-values are calculated by Student’s t-test