Fig. 3
An increase in p53 levels is not sufficient in modulating p53-binding kinetics but p53-CTD acetylation is also necessary. a Western blot of HaloTag-p53 total protein and acetylated at lysine 382 upon exposure to 10 Gy IR or Doxycycline. Quantifications of western blots are provided in Supplementary Fig. 4. b Complement cumulative distribution of single-molecule displacements at different times upon induction of HaloTag-p53 expression by doxycycline and measured bound fraction (inset, 3 replicates, n cells = 25, 19, 25, 9, n displacements = 14 278, 14 420, 10 807, 11 227 for 0 h, 2 h, 4 h, and 24 h after doxycycline, respectively. c Distribution of residence times and average residence times (inset) following doxycycline induction (n bound = 80, 180, 67, 80). d Western blot of HaloTag-p53 total protein and acetylated at lysine 382 upon irradiation and upon the combination of IR and Wortmannin. Quantifications of western blots are provided in Supplementary Fig. 4. e Cumulative distribution of displacements (2 replicates, n cells = 17, 15, 15, n displacements = 17 592, 9846, 17 272 for Ctrl, IR, IR+Wort, respectively) and f the complement cumulative distribution of residence times (n bound = 115, 107, 172) upon irradiation and treatment with Wortmannin (error bars: SD for bound fractions, 95% CI for average residence times)
