Table 1 In vitro properties of purified GCaMP6 and MatryoshCaMP6s calcium sensors

From: Ratiometric Matryoshka biosensors from a nested cassette of green- and orange-emitting fluorescent proteins

Sensor

K d (nM) ΔF/F0

K d (nM) ΔR/R0

pK a,apo

pK a,sat

Dynamic range440 exc ΔR/R0

Dynamic range440 exc ΔF/F0

Dynamic range485 exc ΔF/F0

GCaMP6s

175 ± 17

 

~ 8a

6.13 ± 0.03

 

9.8 ± 0.3

49.7 ± 0.4

MatryoshCaMP6s

197 ± 23

196 ± 22

~ 8a

6.09 ± 0.03

8.5 ± 0.2

8.6 ± 0.1

41.8 ± 0.9

sfGCaMP6s

481 ± 45

 

8.26 ± 0.01

6.01 ± 0.03

 

9.5 ± 1.1

12.7 ± 0.2

sfMatryoshCaMP6s

501 ± 64

483 ± 51

8.14 ± 0.01

6.03 ± 0.03

7.6 ± 0.3

8.1 ± 0.5

9.1 ± 0.4

sfGCaMP6s-T78H

303 ± 28

 

8.47 ± 0.01

5.78 ± 0.02

 

11.4 ± 0.5

19.3 ± 2.8

sfMatryoshCaMP6s-T78H

271 ± 10

265 ± 8

8.34 ± 0.02

5.74 ± 0.03

11.9 ± 0.6

12.1 ± 0.5

16.4 ± 0.8

  1. Calcium affinities and pKa values retrieved from the calcium and pH titration fits in Fig. 2c and Supplementary Fig. 5, respectively
  2. aValues are estimates, since saturation was not reached at the maximal pH tested here (pH 10.5)
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