Table 1 Composition of the culture medium at each stage
Stage | Expansion | Diff1 | Diff2 | Diff3 | Diff4 | Diff5 |
|---|---|---|---|---|---|---|
Day | D1–D5 | D5–D9 | D9–D12 | D12–D16 | D16–D20 | D20–D23 |
Density | 1E5/ml | 1E5/ml | Dilute into 4X | 1E5/ml | 1E6/ml | 5E6/ml |
Medium | Stemspan | IMDM | IMDM | IMDM | IMDM | IMDM |
| Â | rhFLT3 | Plasma | Plasma | Plasma | Plasma | Plasma |
| Â | rhSCF | AB serum | AB serum | AB serum | AB serum | AB serum |
| Â | rhIL-3 | Heparin | Heparin | Heparin | Heparin | Heparin |
| Â | rhIL-6 | Insulin | Insulin | Insulin | Insulin | Insulin |
| Â | Dex | Holo-transferrin | Holo-transferrin | Holo-transferrin | Holo-transferrin | Holo-transferrin |
| Â | Â | SCF | SCF | SCF | Epo | Â |
| Â | Â | IL-3 | IL-3 | Epo | Â | Â |
| Â | Â | Epo | Epo | Â | Â | Â |
