Fig. 3
Development and validation of the BiAD sensor 2. a Representative fluorescence microscopy image showing that BiAD sensor 2 gives rise to distinct BiFC signals in HEK293 cells. NLS-mRuby2 was used as transfection control. b Co-localization of the BiFC signal with the TALE protein fused to mRuby2 documents the high-DNA specificity of the BiAD sensor. c Loss of the BiFC signal when the R44Q MBD variant was used as a detector module confirms the 5mC specificity of the BiAD sensor. d Quantification of the experiments representatively shown in panels a–c. The error bars represent the s.e.m. for two biological repeats (for details cf. Methods and Supplementary Tables 2, 6, and 7). All cells were fixed at 48 h after transfection. Scale bar for all images is 10 µm
