Fig. 2
The primary modification and degradation sites differ for WT and mutant Blimp-1 proteins. a Flag-tagged WT or mutant Blimp-1-expressing plasmids were co-transfected with empty or SENP1-expressing plasmid into 293T cells, and then cells were treated with or without MG132 for 24 h. The lysates were immunoprecipitated (IP) with anti-flag antibody and then immunoblotted with antibodies against Sumo1, Sumo-2/3, and Ub. b 293T cells expressing GFP-tagged WT or mutant Blimp-1 proteins were treated with MG132 or left untreated for 12 h and observed under a confocal microscope. Scale bar: 5 μm. HeLa cells co-expressing GFP-tagged WT/mutant Blimp-1 proteins with mCherry-Sumo1 (c), mCherry-Sumo-3 (d), or mCherry-Ub (e) were treated with MG132 for 12 h and observed under a confocal microscope. Scale bar: 7.5 μm
