Fig. 1
From: Prophage-triggered membrane vesicle formation through peptidoglycan damage in Bacillus subtilis

MVs are released by B. subtilis 168 at the onset of cell death. MV formation was followed at 30 min intervals at room temperature. Membranes were stained with the red fluorescent dye FM4-64 and SYTOX green was used to visualize dead cells and extracellular DNA (green). White arrows indicate cells releasing MVs and green arrows indicate extracellular DNA. Scale bar, 5 μm