Fig. 6 | Nature Communications

Fig. 6

From: The cytoplasmic nuclear receptor RARγ controls RIP1 initiated cell death when cIAP activity is inhibited

Fig. 6

RARγ is involved in death receptor and genotoxic stresses-induced cell death. a Cell death analysis of HT-29 cont-shRNA and RARγ-shRNA-A treated with DMSO, TSZ, TRAIL + SZ, CH11 + SZ or FasL + SZ for 24 h (left panel), or treated with DMSO, TRAIL + S, CH11 + S or FasL + S for 24 h (right panel). Cell death was determined by PI staining using flow cytometry. (*P < 0.05 versus cont-shRNA; ANOVA). The bars represent the mean ± s.e.m. of three experiments. b HT-29 cont-shRNA, and RARγ-shRNA-A treated with FasL + SZ for the indicated times. Cell lysates were immunoprecipitated with anti-Caspase-8 antibody and analyzed with the indicated antibodies. Blots are representative of one of the three experiments

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