Fig. 7

LXRs downregulate intrinsic invasiveness properties of PTEN-negative tumor cells. a Boyden chamber assays performed using PC3 cells transduced with SULT2B1b or Mock construct. Migrated cells are stained with hematoxylin staining (N = 8), scale bar 100 µm. b Description for renal subcapsular implantation in immunocompromized host mice (Nude mice) of prostate specimen from Pten ^{pc −/−} and Pten ^{pc −/−} lxrαβ ^−/− mice. c, d Representative histological features (HE) and Ki67 staining of implant after 1 month of growth, and quantification of Ki67-positive cells from Pten ^{pc −/−} (N = 6) and Pten ^{pc −/−} lxrαβ ^−/− (N = 6) grafts on Nude mice, scale bar 100 µm. e Evaluation of metastatic dissemination on lumbar lymph nodes and lung using prostatic markers CK18 and PSCA in grafted nude mice (N = 6 per group). Nuclei are stained using Hoescht (blue), scale bar 100 µm. f Recapitulative table of metastatic phenotype in lungs and lumbar lymph nodes of grafted Nude mice. All data are represented as mean ± SEM and statistical analyses were performed with the Student’s t-test; ***p < 0.001