Fig. 8
From: Caspase-1 cleaves PPARγ for potentiating the pro-tumor action of TAMs
tPPARγΔ64D regulates TAM differentiation and metabolic reprogramming through MCAD. a–c LacZ overexpressing THP-1 macrophages were cultured alone or cocultured with MCF-7 for 2 d, THP-1 macrophages overexpressing tPPARγΔ64D or simultaneously overexpressing tPPARγΔ64D and MCAD were cocultured with MCF-7 for 2 d, TAM hallmarks were analyzed by RT-qPCR (a) and lipid content was analyzed by Oil Red O staining (b) PPARγ and MCAD expression were analyzed by immunoblot (c). d–f LacZ overexpressing THP-1 macrophages were cultured alone or cocultured with MCF-7 for 2 days, MCAD−/− THP-1 macrophages overexpressing tPPARγΔ64D or LacZ were cocultured with MCF-7 for 2 d, TAM hallmarks were analyzed by RT-qPCR (d) and lipid content was analyzed by Oil Red O staining (e), PPARγ and MCAD expression were analyzed by immunoblot (f). g LacZ overexpressing THP-1 cells were cultured alone or cocultured with MCF-7 in the absence or presence of YVAD for 2 d, MCAD knockout THP-1 macrophages, tPPARγ overexpression THP-1 macrophages (wild type PPARγ was deleted) and MCAD overexpression THP-1 macrophages were cocultured with MCF-7 for 2 d. Flow cytometry analysis of macrophage phagocytic index (phagocytosis of latex beads). MFI represents mean fluorescence intensity. h Flow cytometry analysis of macrophage (described in a–c) phagocytic index. i Flow cytometry analysis of macrophage (described in d–f) phagocytic index. All the histograms in this figure show means ± s.e.m. (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, ns, not significant, one way ANOVA for multiple comparisons)
