Fig. 4 | Nature Communications

Fig. 4

From: Genetic and epigenetic features direct differential efficiency of Xist-mediated silencing at X-chromosomal and autosomal locations

Fig. 4

Xist-mediated rescue of ESC clones from lethal aneuploidy. a Neuronal differentiation of Tg-X;8 and Tg-X clones. Representative bright field images of live cells at day 2, 4, 6, and 8 of neuronal differentiation in doxycycline-treated and -untreated conditions are shown. Scale bars represent 100 μm. b Xist allele-specific qPCR analysis of Tg-X;8 (top), Tg-X (middle), and Tg-E (bottom) clones upon neuronal differentiation. Day 0 allele-specific analysis refers to total Xist expression levels shown in Supplementary Fig. 5. The mean and SD of three to four independent experiments are shown. c, d RNA-seq analysis. Box plots showing Cast/all ratio of chromosomes X c and chromosome 8 d genes in Tg-X;8 clones at day 0 and day 14 of neuronal differentiation in the absence of doxycycline. Data of two independent experiments are shown. e RNA-seq analysis. Box plot showing Cast/all ratio of chromosome X genes at day 0 and 14 of neuronal differentiation of wild-type F1 2-1 cells

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